PDE6D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38346

Novus Biologicals, part of Bio-Techne
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NBP2-38346
NBP2-38346-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDE6D Antibodies »

Product Summary for PDE6D Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE6D Antibody

Western Blot: PDE6D Antibody [NBP2-38346]

Western Blot: PDE6D Antibody [NBP2-38346]

Western Blot: PDE6D Antibody [NBP2-38346] - Analysis in control (vector only transfected HEK293T lysate) and PDE6D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PDE6D Antibody [NBP2-38346]

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP2-38346]

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP2-38346] - Staining of human prostate shows moderate to strong positivity in plasma membrane in glandular cells.
Western Blot: PDE6D Antibody [NBP2-38346]

Western Blot: PDE6D Antibody [NBP2-38346]

Western Blot: PDE6D Antibody [NBP2-38346] - Analysis in human cell lines SK-MEL-30 and Caco-2 using anti-PDE6D antibody. Corresponding PDE6D RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
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Applications for PDE6D Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE6D

PDE6D is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha® (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha®/alpha® homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.

Long Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

Alternate Names

GMP-PDE delta, PDED, Protein p17

Gene Symbol

PDE6D

UniProt

O43924 (Human)

Additional PDE6D Products

Product Documents for PDE6D Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDE6D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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