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PDE6G Antibody (OTI2F5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73298

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human PDE6G(NP_002593) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

9.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE6G Antibody (OTI2F5) - Azide and BSA Free

Western Blot: PDE6G Antibody (OTI2F5)Azide and BSA Free [NBP2-73298]

Western Blot: PDE6G Antibody (OTI2F5)Azide and BSA Free [NBP2-73298]

Western Blot: PDE6G Antibody (OTI2F5) - Azide and BSA Free [NBP2-73298] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE6G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE6G.
Immunohistochemistry: PDE6G Antibody (OTI2F5) - Azide and BSA Free [NBP2-73298]

Immunohistochemistry: PDE6G Antibody (OTI2F5) - Azide and BSA Free [NBP2-73298]

Immunohistochemistry: PDE6G Antibody (OTI2F5) - Azide and BSA Free [NBP2-73298] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE6G mouse monoclonal antibody.

Applications for PDE6G Antibody (OTI2F5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE6G

PDE6G encodes the gamma subunit of cyclic GMP-phosphodiesterase, which is composed of alpha- and beta- catalytic subunits and two identical, inhibitory gamma subunits. PDE6G is expressed in rod photoreceptors and functions in the phototransduction signaling cascade. PDE6G is also expressed in a variety of other tissues, and has been shown to regulate the c-Src protein kinase and G-protein-coupled receptor kinase 2. Alternative splicing results in multiple transcript variants.

Long Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma

Alternate Names

GMP-PDE gamma, PDEG

Gene Symbol

PDE6G

Additional PDE6G Products

Product Documents for PDE6G Antibody (OTI2F5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDE6G Antibody (OTI2F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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