PDE8A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-643

Novus Biologicals, part of Bio-Techne
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NB300-643
NB300-643-0.025mg
NB300-643-0.05mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all PDE8A Antibodies »

Product Specifications

Immunogen

Synthetic peptide (Human) (C terminal).

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Specificity

PDE8A

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE8A Antibody

Western Blot: PDE8A Antibody [NB300-643]

Western Blot: PDE8A Antibody [NB300-643]

Western Blot: PDE8A Antibody [NB300-643] - Western blot using PDE8A (NB300-641) antibody and PC-PDE8A western blot control.

Applications for PDE8A Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-102 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 50 ul of distilled water to a final concentration is 1.0 mg/ml.

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE8A

Phosphodiesterase type 8 (PDE8) is one of the many phosphodiesterases that compartmentalize and hydrolyze cAMP into AMP. The cAMP-specific PDE8 family is comprised of 2 genes (PDE8A and PDE8B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE8 family is a high affinity cAMP-specific, IBMX sensitive PDE. PDE8 has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE8A protein showed significant homology to other cAMP-dependent PDEs (23%) within the catalytic domain. PDE8A is widely expressed in various tissues in contrast to PDE8B that is exclusively expressed in thyroid gland. The PDE8A transcripts are found in brain, pancreas, placenta, thyroid, spleen, trachea, prostate, and uterus.

Long Name

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Alternate Names

cAMP-specific cyclic nucleotide phosphodiesterase 8A, EC 3.1.4.17, FLJ16150, high affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8A, high-affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8A, HsT19550, phosphodiesterase 8A

Entrez Gene IDs

5151 (Human); 18584 (Mouse); 308776 (Rat)

Gene Symbol

PDE8A

Additional PDE8A Products

Product Documents for PDE8A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDE8A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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