PDGF-B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52533

Novus Biologicals, part of Bio-Techne
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NBP1-52533
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Key Product Details

Species Reactivity

Validated:

Human, Bovine, Canine, Sheep

Cited:

Mouse, Canine

Predicted:

Bat (92%), Equine (92%), Monkey (100%), Mouse (92%), Opossum (100%), Primate (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Block/Neutralize, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PDGF-B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KHRKFKHTHDKT, from the C Terminus of the PDGF-B protein sequence according to NP_002599.1 and NP_148937.1.

Epitope

C-Terminus

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 28883003).Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (92%), Feline (83%).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PDGF-B Antibody

Immunocytochemistry/ Immunofluorescence: PDGF-B Antibody [NBP1-52533]

Immunocytochemistry/ Immunofluorescence: PDGF-B Antibody [NBP1-52533]

Immunocytochemistry/Immunofluorescence: PDGF-B Antibody [NBP1-52533] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533]

Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533]

Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533] - Analysis of anti-PDGFB antibody with human pancreas at concentration 3.75 ug/ml.
Flow Cytometry: PDGF-B Antibody [NBP1-52533]

Flow Cytometry: PDGF-B Antibody [NBP1-52533]

Flow Cytometry: PDGF-B Antibody [NBP1-52533] - Flow cytometric analysis of fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for PDGF-B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

1:128000
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDGF-B

Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimmer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.

Long Name

Platelet-derived Growth Factor B

Alternate Names

C-Sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV

Entrez Gene IDs

5155 (Human)

Gene Symbol

PDGFB

UniProt

P01127

Additional PDGF-B Products

Product Documents for PDGF-B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDGF-B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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