PDGFA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005154-D01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDGF-A Antibodies »

Product Specifications

Immunogen

PDGFA (NP_002598.4, 1 a.a. - 211 a.a.) full-length human protein. MRTLACLLLLGCGYLAHVLAEEAEIPREVIERLARSQIHSIRDLQRLLEIDSVGSEDSLDTSLRAHGVHATKHVPEKRPLPIRRKRSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT

Specificity

PDGFA - platelet-derived growth factor alpha polypeptide,

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for PDGFA Antibody

Western Blot: PDGFA Antibody [H00005154-D01P]

Western Blot: PDGFA Antibody [H00005154-D01P]

Western Blot: PDGFA Antibody [H00005154-D01P] - Analysis of PDGFA expression in transfected 293T cell line by PDGFA polyclonal antibody.Lane 1: PDGFA transfected lysate(24.00 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: PDGFA Antibody [H00005154-D01P]

Proximity Ligation Assay: PDGFA Antibody [H00005154-D01P]

Proximity Ligation Assay: PDGFA Antibody [H00005154-D01P] - Analysis of protein-protein interactions between PDGFA and PDGFRA. HeLa cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PDGFRA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Assay: PDGFA Antibody [H00005154-D01P]

Proximity Ligation Assay: PDGFA Antibody [H00005154-D01P]

Proximity Ligation Assay: PDGFA Antibody [H00005154-D01P] - Analysis of protein-protein interactions between PDGFA and PLCG1. Huh7 cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for PDGFA Antibody

Application
Recommended Usage

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDGF-A

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq]

Long Name

Platelet-derived Growth Factor A

Alternate Names

PDGF-1, PDGF1PDGF A-chain, PDGF-A, Platelet-derived growth factor A chain, platelet-derived growth factor alpha chain, platelet-derived growth factor alpha isoform 2 preproprotein, platelet-derived growth factor alpha polypeptidePDGF subunit A, platelet-derived growth factor subunit A

Entrez Gene IDs

5154 (Human)

Gene Symbol

PDGFA

UniProt

NP_002598.4 (Human)

Additional PDGF-A Products

Product Documents for PDGFA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDGFA Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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