PDGFA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99113
![Western Blot: PDGFA Antibody [NBP2-99113]](https://resources.bio-techne.com/images/products/PDGFA-Antibody-Western-Blot-NBP2-99113-img0002.jpg "Western Blot: PDGFA Antibody [NBP2-99113]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PDGFA Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PDGFA.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PDGFA Antibody
Western Blot: PDGFA Antibody [NBP2-99113]
Western Blot: PDGFA Antibody [NBP2-99113] - Anti-PDGFA rabbit polyclonal antibody at 1:500 dilution. Lane A: U937 Whole Cell Lysate. Lane B: HL60 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa![Immunoprecipitation: PDGFA Antibody [NBP2-99113]](https://resources.bio-techne.com/images/products/PDGFA-Antibody-Immunoprecipitation-NBP2-99113-img0003.jpg "Immunoprecipitation: PDGFA Antibody [NBP2-99113]")
Immunoprecipitation: PDGFA Antibody [NBP2-99113]
Immunoprecipitation: PDGFA Antibody [NBP2-99113] - PDGFA was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-PDGFA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-PDGFA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDaApplications for PDGFA Antibody
Application
Recommended Usage
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PDGF-A
Long Name
Platelet-derived Growth Factor A
Alternate Names
PDGF-1, PDGF1PDGF A-chain, PDGF-A, Platelet-derived growth factor A chain, platelet-derived growth factor alpha chain, platelet-derived growth factor alpha isoform 2 preproprotein, platelet-derived growth factor alpha polypeptidePDGF subunit A, platelet-derived growth factor subunit A
Gene Symbol
PDGFA
Additional PDGF-A Products
Product Documents for PDGFA Antibody
Product Specific Notices for PDGFA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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