PDK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32581

Novus Biologicals, part of Bio-Techne
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NBP1-32581
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Squirrel, Xenopus

Cited:

Human, Mouse

Predicted:

Bovine (98%), Chicken (93%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDK3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary.

Reactivity Notes

Frog (100%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDK3 Antibody

Western Blot: PDK3 Antibody [NBP1-32581]

Western Blot: PDK3 Antibody [NBP1-32581]

Western Blot: PDK3 Antibody [NBP1-32581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PDK3 Antibody [NBP1-32581]

Immunocytochemistry/ Immunofluorescence: PDK3 Antibody [NBP1-32581]

Immunocytochemistry/Immunofluorescence: PDK3 Antibody [NBP1-32581] - Methanol-fixed HepG2, using PDK3(NBP1-32581) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581]

Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581]

Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581] - Ca922 xenograft, using PDK3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
View 2 more images

Applications for PDK3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-32581 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDK3

PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Long Name

[Pyruvate dehydrogenase

Alternate Names

PDHK3

Entrez Gene IDs

5165 (Human)

Gene Symbol

PDK3

UniProt

Q15120 (Human)

Additional PDK3 Products

Product Documents for PDK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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