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PDLIM1 Antibody (CPTC-PDLIM1-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07518

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-PDLIM1-1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant human full-length PDLIM1 protein (Uniprot: O00151)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08842)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PDLIM1 Antibody (CPTC-PDLIM1-1)

Immunohistochemistry-Paraffin: PDLIM1 Antibody (CPTC-PDLIM1-1) [NBP3-07518]

Immunohistochemistry-Paraffin: PDLIM1 Antibody (CPTC-PDLIM1-1) [NBP3-07518]

Immunohistochemistry-Paraffin: PDLIM1 Antibody (CPTC-PDLIM1-1) [NBP3-07518] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with PDLIM1 Mouse Monoclonal Antibody (PDLIM-1).

Applications for PDLIM1 Antibody (CPTC-PDLIM1-1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PDLIM1

CLP36 is a PDZ-domain and LIM-domain containing protein that associates with actin filaments and stress fibers via alpha-actinin. CLP36 has been show to be critical to stress fiber formation and focal adhesions. Alternative names for CLP36 include Elfin, C-terminal LIM domain protein 1, PDZ and LIM domain protein 1, CLIM1, and PDLIM1.

Alternate Names

CLIM1carboxyl terminal LIM domain protein 1, CLP-36, CLP36carboxy terminal LIM domain protein 1, C-terminal LIM domain protein 1, elfin, enigma, hCLIM1, LIM domain protein CLP-36, PDZ and LIM domain 1, PDZ and LIM domain 1 (elfin), PDZ and LIM domain protein 1

Gene Symbol

PDLIM1

UniProt

Additional PDLIM1 Products

Product Documents for PDLIM1 Antibody (CPTC-PDLIM1-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDLIM1 Antibody (CPTC-PDLIM1-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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