PDLIM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85800

Novus Biologicals, part of Bio-Techne
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NBP1-85800-25ul
NBP1-85800
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDLIM2 Antibodies »

Product Summary for PDLIM2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPGHTNGDSSLEVLATRFQGSVRTYTESQSSLRSSYSSPTSLSPRAGSPFSPPPSSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQAGLGRAGDSAVLVLPPSPGPRSSRPSMDSEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDLIM2 Antibody

Western Blot: PDLIM2 Antibody [NBP1-85800]

Western Blot: PDLIM2 Antibody [NBP1-85800]

Western Blot: PDLIM2 Antibody [NBP1-85800] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: PDLIM2 Antibody [NBP1-85800]

Immunocytochemistry/ Immunofluorescence: PDLIM2 Antibody [NBP1-85800]

Immunocytochemistry/Immunofluorescence: PDLIM2 Antibody [NBP1-85800] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM2 Antibody [NBP1-85800]

Immunohistochemistry-Paraffin: PDLIM2 Antibody [NBP1-85800]

Immunohistochemistry-Paraffin: PDLIM2 Antibody [NBP1-85800] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Applications for PDLIM2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDLIM2

Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for themigratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin andsuppresses anchorage independent growth. May contribute to tumor cell migratory capacity

Alternate Names

FLJ34715, MYSTIQUE, PDZ and LIM domain 2 (mystique), PDZ and LIM domain protein 2, PDZ-LIM protein mystique, SLIM

Gene Symbol

PDLIM2

Additional PDLIM2 Products

Product Documents for PDLIM2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDLIM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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