PDLIM3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19755

Novus Biologicals, part of Bio-Techne
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NBP2-19755
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (93%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDLIM3 Antibodies »

Product Summary for PDLIM3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDLIM3. The exact sequence is proprietary.

Reactivity Notes

Rat (80%), Pig (80%).

Localization

Cytoplasm; myofibril; sarcomere; Z-disk

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDLIM3 Antibody

Western Blot: PDLIM3 Antibody [NBP2-19755]

Western Blot: PDLIM3 Antibody [NBP2-19755]

Western Blot: PDLIM3 Antibody [NBP2-19755] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: PDLIM3 Antibody [NBP2-19755]

Western Blot: PDLIM3 Antibody [NBP2-19755]

Western Blot: PDLIM3 Antibody [NBP2-19755] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 10% SDS PAGE gel, diluted at 1:1000.
PDLIM3 Antibody

Western Blot: PDLIM3 Antibody [NBP2-19755] -

Western Blot: PDLIM3 Antibody [NBP2-19755] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody (NBP2-19755) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PDLIM3 Antibody

Application
Recommended Usage

Western Blot

1:500-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDLIM3

The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Actinin-associated LIM protein, ALPFLJ26096, Alpha-actinin-2-associated LIM protein, DKFZp686L0362, enigma homolog, PDZ and LIM domain 3, PDZ and LIM domain protein 3

Gene Symbol

PDLIM3

Additional PDLIM3 Products

Product Documents for PDLIM3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDLIM3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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