PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73332

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3H3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PDX-1/IPF1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDX1 (NP_000200) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free

Western Blot: PDX-1/IPF1 Antibody (OTI3H3)Azide and BSA Free [NBP2-73332]

Western Blot: PDX-1/IPF1 Antibody (OTI3H3)Azide and BSA Free [NBP2-73332]

Western Blot: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .
Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332]

Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332]

Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Human Kidney tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,2) (1:150)
Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332]

Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332]

Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDX-1/IPF1

PDX1 (Pancrease/duodenum homeobox 1, Insulin promoter factor 1, IPF1, Islet/duodenum homeobox 1, IDX1, Somatatostatin transactivating factor 1, STF1, Insulin upstream factor 1, UUF1, Glucose-sensitive factor, GSF) activates transcription of the insulin and somatostain genes. PDX1 is required for maintaining the hormone-producing phenotype of the beta-cell.

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Gene Symbol

PDX1

Additional PDX-1/IPF1 Products

Product Documents for PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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