PEA-15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88031

Novus Biologicals, part of Bio-Techne
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NBP2-88031
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

0.5 mg/ml

View all PEA-15 Antibodies »

Product Summary for PEA-15 Antibody

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human PEA-15. Peptide sequence: DYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for PEA-15 Antibody

Western Blot: PEA-15 Antibody [NBP2-88031]

Western Blot: PEA-15 Antibody [NBP2-88031]

Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain
Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-88031]

Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-88031]

Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-88031] - Rabbit Anti-PEA15 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:100.
Western Blot: PEA-15 Antibody [NBP2-88031]

Western Blot: PEA-15 Antibody [NBP2-88031]

Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody. Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate. Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP. Secondry Antibody Dilution : 1:50

Applications for PEA-15 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PEA-15

PEA-15 (phosphoprotein enriched in astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 co-localizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle. PEA-15 is also referred to as PED, phosphoprotein enriched in diabetes, for its elevated expression in type 2 diabetic patients.

Long Name

Phosphoprotein Enriched in Astrocytes 15

Alternate Names

HMAT1, HUMMAT1H, MAT1H, PEA15, PED

Gene Symbol

PEA15

Additional PEA-15 Products

Product Documents for PEA-15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PEA-15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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