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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide mapping to a region between residues 1000 and 1050 of human, proline-, glutamic acid-, leucine-rich protein 1/ modulator of nongenomic activity of estrogen receptor using the numbering given in entry AAN41255.1 (GeneID 27043).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PELP1 Antibody

Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331]

Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331]

Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.
Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331]

Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for PELP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2500-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PELP1

PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004).[supplied by OMIM]

Alternate Names

HMX3, MNARmodulator of nongenomic activity of estrogen receptor, Modulator of non-genomic activity of estrogen receptor, P160, proline and glutamic acid rich nuclear protein, proline, glutamate and leucine rich protein 1, proline, glutamic acid and leucine rich protein 1, proline-, glutamic acid- and leucine-rich protein 1, proline-, glutamic acid-, leucine-rich protein 1, Transcription factor HMX3

Entrez Gene IDs

27043 (Human)

Gene Symbol

PELP1

Additional PELP1 Products

Product Documents for PELP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PELP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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