Perforin Antibody (CB5.4) [Janelia Fluor® 646]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97512JF646

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-97512AF350

Alexa Fluor 405

NBP1-97512AF405

Alexa Fluor 488

NBP1-97512AF488

Alexa Fluor 532

NBP1-97512AF532

Alexa Fluor 594

NBP1-97512AF594

Alexa Fluor 647

NBP1-97512AF647

Alexa Fluor 700

NBP1-97512AF700

Alexa Fluor 750

NBP1-97512AF750

Biotin

NBP1-97512B

CoraFluor 1

NBP1-97512CL1

DyLight 350

NBP1-97512UV

DyLight 405

NBP1-97512V

DyLight 488

NBP1-97512G

DyLight 550

NBP1-97512R

DyLight 594

NBP1-97512DL594

DyLight 650

NBP1-97512C

DyLight 680

NBP1-97512FR

DyLight 755

NBP1-97512IR

FITC

NBP1-97512F

HRP

NBP1-97512H

Janelia Fluor 525

NBP1-97512JF525

Janelia Fluor 549

NBP1-97512JF549

Janelia Fluor 669

NBP1-97512JF669

Forumulation

Catalog #

BSA Free

NBP1-97512

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Janelia Fluor 646

Antibody Source

Monoclonal Rat IgG_2A Clone # CB5.4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Perforin Antibodies »

Product Specifications

Immunogen

Recombinant mouse perforin (aa 98-534).

Specificity

Recognizes mouse perforin (region aa 402-534).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Perforin Antibody (CB5.4) [Janelia Fluor® 646]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Perforin Antibody (CB5.4) [Janelia Fluor® 646]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Perforin Antibody (CB5.4) [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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