Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CE2.10

Format

BSA Free

Concentration

1.0 mg/ml

View all Perforin Antibodies »

Product Specifications

Immunogen

Recombinant mouse perforin (aa 98-534).

Reactivity Notes

Immunoprecipitation (Human and Mouse), Western Blot (Mouse only). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Recognizes an epitope in the region aa 402-534 of human and mouse perforin. Does NOT detect Human in Western Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Perforin Antibody (CE2.10) - BSA Free

Western Blot: Perforin Antibody (CE2.10)BSA Free [NBP1-97440]

Western Blot: Perforin Antibody (CE2.10)BSA Free [NBP1-97440]

Western Blot: Perforin Antibody (CE2.10) [NBP1-97440] - Detectioon of perforin in the mouse T cell clone B6.1. (lane 2). As control cell line HEK 293 was used (lane 1). The protein migrates around ~66kDa.Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10. antibody at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Applications for Perforin Antibody (CE2.10) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody is useful in Immunoprecipitation (Human and Mouse) and Western Blot (Mouse ONLY).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Perforin Antibody (CE2.10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Perforin Antibody (CE2.10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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