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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # PRF1/2470

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human Perforin protein fragment (around aa 413-552) (exact sequence is proprietary) (Uniprot: P14222)

Localization

Cytoplasmic

Marker

Apoptosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79751).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Perforin Antibody (PRF1/2470) - Azide and BSA Free

Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/2470) - Azide and BSA Free [NBP2-79886]

Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/2470) - Azide and BSA Free [NBP2-79886]

Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/2470) - Azide and BSA Free [NBP2-79886] - Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Monospecific Mouse Monoclonal Antibody (PRF1/2470).
Protein Array: Perforin Antibody (PRF1/2470) - Azide and BSA Free [NBP2-79886]

Protein Array: Perforin Antibody (PRF1/2470) - Azide and BSA Free [NBP2-79886]

Protein Array: Perforin Antibody (PRF1/2470) - Azide and BSA Free [NBP2-79886] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Applications for Perforin Antibody (PRF1/2470) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Perforin Antibody (PRF1/2470) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Perforin Antibody (PRF1/2470) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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