Perforin Antibody (PRF1/7077R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13751
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/7077R) [NBP3-13751]](https://resources.bio-techne.com/images/products/Perforin-Antibody-PRF1-7077R-Immunohistochemistry-Paraffin-NBP3-13751-img0001.jpg "Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/7077R) [NBP3-13751]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # PRF1/7077R
Concentration
0.2 mg/ml
Product Summary for Perforin Antibody (PRF1/7077R)
Immunogen
Recombinant human Perforin protein fragment (around aa 413-552) (exact sequence is proprietary) (Uniprot: P14222)
Localization
Cytoplasm
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14154)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Perforin Antibody (PRF1/7077R)
Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/7077R) [NBP3-13751]
Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/7077R) [NBP3-13751] - Formalin-fixed, paraffin-embedded human spleen stained with Perforin antibody (PRF1/7077R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Perforin Antibody (PRF1/7077R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Perforin
Long Name
Perforin 1 (Pore Forming Protein)
Alternate Names
Cytolysin, FLH2, HPLH2, P1, PFP, PRF1
Gene Symbol
PRF1
UniProt
Additional Perforin Products
Product Documents for Perforin Antibody (PRF1/7077R)
Product Specific Notices for Perforin Antibody (PRF1/7077R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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