Pericentrin Antibody

Immunohistochemistry: Pericentrin Antibody [NB100-61071]

Immunohistochemistry: Pericentrin Antibody [NB100-61071] - Immunofluorescent analysis of cilia in renal tissues. Sections (4 um) of renal parenchymal tissues and tumor tissues were stained with DAPI, acetylated-alpha-tubulin (Ac-tub) and pericentrin (PCNT) to mark cell nuclei and cilia. Presented images are maximal projections of confocal images of typical parenchymal tissue and a representative ccRCC. Scale bars 20 um. Statistics were determined by performing a paired t-test at a 95% confidence interval. Image collected and cropped by CiteAb from the following publication (https://ciliajournal.biomedcentral.com/articles/10.1186/2046-2530-2-2) licensed under a CC-BY license.

Western Blot: Pericentrin Antibody [NB100-61071]

Western Blot: Pericentrin Antibody [NB100-61071] - Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrinantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 second.

Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071]

Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 594 Conjugated used at a dilution of 1:100. Counterstain. DAPI (blue) and Phalloidin (green)