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Pericentrin Antibody - C-terminus

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63208

Novus Biologicals, part of Bio-Techne
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NB100-63208

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide sequence C-ARAIIREGSLEGS corresponding to the C-terminal of pericentrin 1 (NP_079120)

Reactivity Notes

Predicted cross-reactivities: Mouse, Rat

Localization

Nuclear

Specificity

NB100-63208 recognizes the C-terminal of human pericentrin 1, a 75 kDa component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localized protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Marker

Centrosome Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pericentrin Antibody - C-terminus

Western Blot: Pericentrin Antibody [NB100-63208]

Western Blot: Pericentrin Antibody [NB100-63208]

Western Blot: Pericentrin Antibody [NB100-63208] - Staining of HeLa lysate (35 ug protein in RIPA buffer) using Goat anti Human pericentrin 1 antibody at 1.0 ug/mL. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Applications for Pericentrin Antibody - C-terminus

Application
Recommended Usage

ELISA

>1:32000

Western Blot

1 - 3 ug/mL

Reviewed Applications

Read 1 review rated 1 using NB100-63208 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pericentrin

Pericentrin is encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Alternate Names

Kendrin, KIAA0402, MOPD2, PCN, PCNT2pericentrin B, PCNTB, pericentrin, pericentrin 2 (kendrin), pericentrin, kendrin (KIAA0402)10KENPCTN2, pericentrin-2, pericentrin-380, pericentrin-B, SCKL4kendrin

Entrez Gene IDs

79902 (Human)

Gene Symbol

NUP85

UniProt

Additional Pericentrin Products

Product Documents for Pericentrin Antibody - C-terminus

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pericentrin Antibody - C-terminus

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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