Perilipin Antibody (1K4W3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16480

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16480-100ul
NBP3-16480-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1K4W3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Perilipin Antibodies »

Product Summary for Perilipin Antibody (1K4W3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Perilipin (O60240). HYVPLPRLSLMEPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRVSDSF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Perilipin Antibody (1K4W3)

Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480]

Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480]

Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480] - Western blot analysis of extracts of various cell lines, using Perilipin Rabbit mAb (NBP3-16480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded rat brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded mouse brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Perilipin Antibody (1K4W3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Perilipin

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody (1K4W3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Perilipin Antibody (1K4W3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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