Periostin/OSF-2 Antibody (CL12090)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21207

Novus Biologicals, part of Bio-Techne
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NBP3-21207-100ul
NBP3-21207-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL12090

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Periostin/OSF-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Synthetic Peptide of Q15063. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Periostin/OSF-2 Antibody (CL12090)

Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody [NBP3-21207] -

Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody [NBP3-21207] -

Staining of U-138 cells using the Anti-POSTN monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-21207] -

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-21207] -

Staining of human colon shows strong positivity in extracellular matrix, as well as moderate positivity in glandular cells.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-21207] -

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP3-21207] -

Analysis in human fallopian tube and liver tissues using NBP3-21207 antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.
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Applications for Periostin/OSF-2 Antibody (CL12090)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (CL12090)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Periostin/OSF-2 Antibody (CL12090)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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