Periostin/OSF-2 Antibody (S03-7E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20049

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-7E3

Concentration

0.3 mg/ml

View all Periostin/OSF-2 Antibodies »

Product Summary for Periostin/OSF-2 Antibody (S03-7E3)

Immunogen

A synthetic peptide of human Periostin/OSF-2 (Uniprot # Q15063)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Periostin/OSF-2 Antibody (S03-7E3)

Western Blot: Periostin/OSF-2 Antibody (S03-7E3) [NBP3-20049]

Western Blot: Periostin/OSF-2 Antibody (S03-7E3) [NBP3-20049]

Western Blot: Periostin/OSF-2 Antibody (S03-7E3) [NBP3-20049] - Western blot detection of Periostin/OSF-2 in C6 cell lysates using NBP3-20049 (1:1000 diluted). Predicted band size: 93kDa. Observed band size: 93kDa.
Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (S03-7E3) [NBP3-20049]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (S03-7E3) [NBP3-20049]

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (S03-7E3) [NBP3-20049] - Immunohistochemistry of Periostin/OSF-2 in paraffin-embedded Human Cholangiocarcinoma using NBP3-20049 at dilution 1/50

Applications for Periostin/OSF-2 Antibody (S03-7E3)

Application
Recommended Usage

Immunohistochemistry

1/200

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (S03-7E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Periostin/OSF-2 Antibody (S03-7E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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