Peripherin Antibody (OTI1E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45406

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E10

Concentration

1 mg/ml

View all Peripherin Antibodies »

Product Summary for Peripherin Antibody (OTI1E10)

Immunogen

Full length human recombinant protein of human PRPH (NP_006253) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peripherin Antibody (OTI1E10)

Western Blot: Peripherin Antibody (OTI1E10) [NBP2-45406]

Western Blot: Peripherin Antibody (OTI1E10) [NBP2-45406]

Western Blot: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peripherin.
Immunohistochemistry: Peripherin Antibody (OTI1E10) [NBP2-45406]

Immunohistochemistry: Peripherin Antibody (OTI1E10) [NBP2-45406]

Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Peripherin Antibody (OTI1E10) [NBP2-45406]

Immunohistochemistry: Peripherin Antibody (OTI1E10) [NBP2-45406]

Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for Peripherin Antibody (OTI1E10)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Peripherin

Peripherin is a Class III intermediate filament subunit found in both the peripheral and central nervous systems, though it is concentrated--as its name suggests--in the neurons of peripheral ganglia and their processes. Antibodies to peripherin can be used in identifying, classifying, and studying neurons in the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease and some neuronally derived tumors. Peripherin is also a major component of the cytoskeleton of PC12 cells, a widely used model system for studies of neuronal differentiation. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.

Alternate Names

Neurofilament 4, neurofilament 4 (57kD), peripherin, PRPH1NEF4

Gene Symbol

PRPH

Additional Peripherin Products

Product Documents for Peripherin Antibody (OTI1E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Peripherin Antibody (OTI1E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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