PERK Antibody (PSH0-81)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32738
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH0-81
Concentration
1 mg/ml
Product Summary for PERK Antibody (PSH0-81)
Immunogen
Recombinant protein within human PERK aa 51-450 / 1,116. (Uniprot: Q9NZJ5)
Localization
Endoplasmic reticulum membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PERK Antibody (PSH0-81)
Western Blot: PERK Antibody (PSH0-81) [NBP3-32738]
Western Blot: PERK Antibody (PSH0-81) [NBP3-32738] - Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (NBP3-32738) at 1/1,000 dilution.Lane 1: MCF7 cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: HEK-293 cell lysate (20 ug/Lane)
Lane 4: HeLa cell lysate (20 ug/Lane)
Lane 5: A549 cell lysate (20 ug/Lane)
Lane 6: U-87 MG cell lysate (20 ug/Lane)
Lane 7: K-562 cell lysate (16 ug/Lane)
Predicted band size: 125 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32738) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for PERK Antibody (PSH0-81)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PERK
Long Name
Eukaryotic Translation Initiation Factor 4B
Alternate Names
EIF2AK3, PEK, WRS
Gene Symbol
EIF2AK3
Additional PERK Products
Product Documents for PERK Antibody (PSH0-81)
Product Specific Notices for PERK Antibody (PSH0-81)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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