Peroxiredoxin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30973
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (95%), Canine (97%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Peroxiredoxin 1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 1. The exact sequence is proprietary.
Reactivity Notes
Chicken (86%), Zebrafish (80%).
Localization
Cytoplasm, Melanosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Peroxiredoxin 1 Antibody
Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973]
Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-30973]
Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-30973] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PRX I stained by PRX I antibody [N1C2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI.Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-30973]
Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-30973] - Mouse lung. PRX I antibody [N1C2] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15minApplications for Peroxiredoxin 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Peroxiredoxin 1
Alternate Names
NKEF-A, PRDX1, PRX1, TDPX2
Gene Symbol
PRDX1
Additional Peroxiredoxin 1 Products
Product Documents for Peroxiredoxin 1 Antibody
Product Specific Notices for Peroxiredoxin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.