Peroxiredoxin 6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90332

Novus Biologicals, part of Bio-Techne
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NBP1-90332
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Peroxiredoxin 6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPA

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20647035)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Peroxiredoxin 6 Antibody

Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332]

Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332]

Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332] - Analysis in human cell lines PC-3 and MCF-7 using anti-PRDX6 antibody. Corresponding PRDX6 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody [NBP1-90332]

Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody [NBP1-90332]

Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody [NBP1-90332] - Staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.
Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332]

Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332]

Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Applications for Peroxiredoxin 6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 6

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Alternate Names

aiPLA2, AOP2, NSGPx, PRDX6

Gene Symbol

PRDX6

Additional Peroxiredoxin 6 Products

Product Documents for Peroxiredoxin 6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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