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Pet1 Antibody (FEV/7311) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23997

Novus Biologicals, part of Bio-Techne
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NBP3-23997

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FEV/7311

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Pet1 Antibody (FEV/7311) - Azide and BSA Free

Immunogen

Recombinant fragment (around aa1-200) of human Pet1 protein (exact sequence is proprietary)

Localization

Cell junction. Cell membrane. Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for Pet1 Antibody (FEV/7311) - Azide and BSA Free

Immunohistochemistry-Paraffin: Pet1 Antibody (FEV/7311) - Azide and BSA Free [NBP3-23997] -

Formalin-fixed, paraffin-embedded human kidney stained with Pet1 antibody (FEV/7311) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Immunohistochemistry-Paraffin: Pet1 Antibody (FEV/7311) - Azide and BSA Free [NBP3-23997] -

Formalin-fixed, paraffin-embedded human thyroid stained with Pet1 antibody (FEV/7311) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Pet1 Antibody (FEV/7311) - Azide and BSA Free [NBP3-23997] -

Formalin-fixed, paraffin-embedded human pancreas stained with Pet1 antibody (FEV/7311) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Pet1 Antibody (FEV/7311) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Pet1

Pet1 belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations.

Alternate Names

FEV (ETS oncogene family), FEV (fifth Ewing variant), Fifth Ewing variant protein, HSRNAFEV, PC12 ETS domain-containing transcription factor 1, PC12 ETS factor 1, PET1, Pet-1, protein FEV

Gene Symbol

FEV

Additional Pet1 Products

Product Documents for Pet1 Antibody (FEV/7311) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pet1 Antibody (FEV/7311) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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