PF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81671

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human, Avian - Chicken

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 954 and 1004 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22986495).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PF1 Antibody

Western Blot: PF1 Antibody [NB100-81671]

Western Blot: PF1 Antibody [NB100-81671]

Western Blot: PF1 Antibody [NB100-81671] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.
Immunoprecipitation: PF1 Antibody [NB100-81671]

Immunoprecipitation: PF1 Antibody [NB100-81671]

Immunoprecipitation: PF1 Antibody [NB100-81671] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81671 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81670. For blotting immunoprecipitated PF1, NB100-81671 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Chip reactivity reported in scientific literature (PMID: 22986495).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PF1

PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks. Alternate names for PF1 include PHF12, PHD finger protein 12, and KIAA1523.

Alternate Names

FLJ34122, KIAA1523MGC131914, Pf1, PHD factor 1, PHD finger protein 12, PHD zinc finger transcription factor

Entrez Gene IDs

57649 (Human); 268448 (Mouse)

Gene Symbol

PHF12

UniProt

Q96QT6 (Human)

Additional PF1 Products

Product Documents for PF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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