PFKFB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45238

Novus Biologicals, part of Bio-Techne
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NBP1-45238
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Key Product Details

Species Reactivity

Human, Bovine, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PFKFB2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SEQNNNSYETKTP-C corresponding to N-Terminus according to NP_006203.2, NP_001018063.1.

Epitope

N-terminus

Reactivity Notes

Expected from sequence similarity: Canine, Bovine

Specificity

This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PFKFB2 Antibody

Immunohistochemistry-Paraffin: PFKFB2 Antibody [NBP1-45238]

Immunohistochemistry-Paraffin: PFKFB2 Antibody [NBP1-45238]

Immunohistochemistry-Paraffin: PFKFB2 Antibody [NBP1-45238] - 2.5ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PFKFB2 Antibody

Application
Recommended Usage

Immunohistochemistry

1ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

detection limit 1:16000
Application Notes
Western blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Spleen.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PFKFB2

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Alternate Names

6PF-2-K/Fru-2,6-P2ase 2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, DKFZp781D2217, fructose-2,6-bisphosphatase, cardiac isozyme, MGC138308,6PF-2-K/Fru-2,6-P2ase heart-type isozyme, MGC138310, PFK/FBPase 2, PFK-2/FBPase-2, PFKFB, cardiac

Entrez Gene IDs

5208 (Human)

Gene Symbol

PFKFB2

UniProt

NP_006203.2 (Human)

Additional PFKFB2 Products

Product Documents for PFKFB2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PFKFB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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