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PGAM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32966

Novus Biologicals, part of Bio-Techne
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NBP1-32966

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Bovine (94%), Mouse (92%), Porcine (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length human PGAM2 Recombinant protein.

Reactivity Notes

Zebrafish (86%), Xenopus laevis (84%).

Specificity

human PGAM2 protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PGAM2 Antibody

Western Blot: PGAM2 Antibody [NBP1-32966]

Western Blot: PGAM2 Antibody [NBP1-32966]

Western Blot: PGAM2 Antibody [NBP1-32966] - A. 50 ug rat muscle lysate/extract 12 % SDS-PAGE PGAM2 antibody dilution: 1:10000
Immunocytochemistry/ Immunofluorescence: PGAM2 Antibody [NBP1-32966]

Immunocytochemistry/ Immunofluorescence: PGAM2 Antibody [NBP1-32966]

Immunocytochemistry/Immunofluorescence: PGAM2 Antibody [NBP1-32966] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGAM2 protein stained by PGAM2 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
PGAM2 Antibody

Western Blot: PGAM2 Antibody [NBP1-32966] -

Western Blot: PGAM2 Antibody [NBP1-32966] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PGAM2 antibody (NBP1-32966) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PGAM2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGAM2

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. (provided by RefSeq)

Alternate Names

BPG-dependent PGAM 2, EC 3.1.3.13, EC 5.4.2.1, EC 5.4.2.4, Muscle-specific phosphoglycerate mutase, PGAM-MGSD10, PGAMMMGC88743, phosphoglycerate mutase 2, phosphoglycerate mutase 2 (muscle), Phosphoglycerate mutase isozyme M

Gene Symbol

PGAM2

Additional PGAM2 Products

Product Documents for PGAM2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGAM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.