PGLYRP2/PGRP-L Antibody

Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-49690]

Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-49690] - Total protein from MCF7 cells, human and mouse kidney were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-PGRP-L in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/ Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-49690]

Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-49690] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGRP-L at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.