PGLYRP3/PGRPIA Antibody (187C434)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56729
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Flow (Cell Surface), Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # 187C434
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 178-193 of human PGRP-1a.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for PGLYRP3/PGRPIA Antibody (187C434)
Western Blot: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729]
Western Blot: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] - Analysis in cell lysates from human brain using this antibody at a dilution of 1:500.Immunocytochemistry/ Immunofluorescence: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729]
Immunocytochemistry/Immunofluorescence: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] - A. HCECs were exposed to HKCA (106 cells/ml) with prior incubation in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kB activation inhibitor quinazoline (NF-kB-I, 10uM) for 1 h. HCECs were treated with 106 cells/ml HKCA for 48 hours in 8-chamber slides and examined by immunofluorescent staining for PGLYRPs 3 and 4. (NB100-56721) C. The percentages of positive cells of PGLYRPs 3 and 4 staining in HCECs in A was quantified. Results shown are the mean +/- SD of four independent experiments; *** p<0.001, as compared with normal control; ^^ p<0.005, ^^^ p<0.001, as compared with HCECs exposed to HKCA. Magnification: 400x (bar = 25um). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0128039) licensed under a CC-BY license.Immunohistochemistry: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] -
NF-kappaB p65 activation was induced by HKCA and inhibited by dectin-1 neutralizing antibody and NF-kappaB activation inhibitor quinazoline (NF-kappaB-I) in HCECs.A). HCECs were exposed to HKCA (106 cells/ml) with prior incubation in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kappaB activation inhibitor quinazoline (NF-kappaB-I, 10uM) for 1 h. HCECs were treated with 106 cells/ml HKCA for 48 hours in 8-chamber slides and examined by immunofluorescent staining for PGLYRPs 2-4. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26039076), licensed under a CC-BY licence.Applications for PGLYRP3/PGRPIA Antibody (187C434)
Application
Recommended Usage
Flow (Cell Surface)
reported in scientific literature (PMID 15839897)
Flow (Intracellular)
reported in scientific literature (PMID 16849490)
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000. Use reported in scientific literature (Uehara et al (2005))
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PGLYRP3/PGRP-I alpha
Long Name
Peptidoglycan Recognition Protein Intermediate, Alpha/Peptidoglycan Recognition Protein 3
Alternate Names
PGRP-I alpha, PGRP1A, PGRPI alpha, PGRPIA
Gene Symbol
PGLYRP3
UniProt
Additional PGLYRP3/PGRP-I alpha Products
Product Documents for PGLYRP3/PGRPIA Antibody (187C434)
Product Specific Notices for PGLYRP3/PGRPIA Antibody (187C434)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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