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PGM3 Antibody (1E2-1B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005238-M01

Novus Biologicals, part of Bio-Techne
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H00005238-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E2-1B12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PGM3 (AAH01258, 1 a.a. ~ 542 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF

Specificity

PGM3 - phosphoglucomutase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PGM3 Antibody (1E2-1B12)

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 2 ug/ml.
Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01]

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi ( Cat # H00005238-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # H00005238-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for PGM3 Antibody (1E2-1B12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PGM3

Interconverts GlcNAc-6-P and GlcNAc-1-P

Alternate Names

AGM1PAGMAcetylglucosamine phosphomutase, DKFZP434B187, EC 5.4.2, EC 5.4.2.3, FLJ11614, FLJ13623, N-acetylglucosamine-phosphate mutase, N-acetylglucosamine-phosphate mutase 1, PGM 3, phosphoacetylglucosamine mutase, phosphoglucomutase 3, Phosphoglucomutase-3

Entrez Gene IDs

5238 (Human)

Gene Symbol

PGM3

OMIM

172100 (Human)

Additional PGM3 Products

Product Documents for PGM3 Antibody (1E2-1B12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGM3 Antibody (1E2-1B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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