PGRMC1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83220
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0026.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Western Blot: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Western-Blot-NBP1-83220-img0015.jpg "Western Blot: PGRMC1 Antibody [NBP1-83220]")
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PGRMC1 Antibody
![Western Blot: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Western-Blot-NBP1-83220-img0029.jpg "Western Blot: PGRMC1 Antibody [NBP1-83220]")
Western Blot: PGRMC1 Antibody [NBP1-83220]
Western Blot: PGRMC1 Antibody [NBP1-83220] - Haem-dependent dimerization of PGRMC1 accelerates tumor growth through the EGFR signaling pathway. Nucleotide sequences of PGRMC1 targeted by shRNA and of the shRNA-resistant full length PGRMC1 expression vector. Stable PGRMC1-knockdown (PGRMC1-KD) HCT116 cells were transiently transfected with the shRNA-resistant expression vector of wild-type PGRMC1 (wt) or the Y113F mutant (Y113F). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11030), licensed under a CC-BY license.![Western Blot: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Western-Blot-NBP1-83220-img0014.jpg "Western Blot: PGRMC1 Antibody [NBP1-83220]")
Western Blot: PGRMC1 Antibody [NBP1-83220]
Western Blot: PGRMC1 Antibody [NBP1-83220] - Analysis in human cell line HEK 293.![Western Blot: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Western-Blot-NBP1-83220-img0028.jpg "Western Blot: PGRMC1 Antibody [NBP1-83220]")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0020.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0021.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0022.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0023.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0024.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-83220-img0025.jpg "Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]")
![Simple Western: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Simple-Western-NBP1-83220-img0005.jpg "Simple Western: PGRMC1 Antibody [NBP1-83220]")
![Simple Western: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Simple-Western-NBP1-83220-img0006.jpg "Simple Western: PGRMC1 Antibody [NBP1-83220]")
![Knockdown Validated: PGRMC1 Antibody [NBP1-83220]](https://resources.bio-techne.com/images/products/PGRMC1-Antibody-Knockdown-Validated-NBP1-83220-img0030.jpg "Knockdown Validated: PGRMC1 Antibody [NBP1-83220]")
Applications for PGRMC1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in literature (PMID:23242527).
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PGRMC1
Long Name
Progesterone Receptor Membrane Component 1
Alternate Names
HPR6.6, MPR, Progesterone Binding Protein
Gene Symbol
PGRMC1
Additional PGRMC1 Products
Product Documents for PGRMC1 Antibody
Product Specific Notices for PGRMC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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