Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PGRMC1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 145 to 195 of human Progesterone Receptor Membrane Component 1 using the numbering given in entry NP_006658.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGRMC1 Antibody - BSA Free

Western Blot: PGRMC1 Antibody [NBP2-36525]

Western Blot: PGRMC1 Antibody [NBP2-36525]

Western Blot: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposuretime of 30 seconds.
Immunohistochemistry: PGRMC1 Antibody [NBP2-36525]

Immunohistochemistry: PGRMC1 Antibody [NBP2-36525]

Immunohistochemistry: PGRMC1 Antibody [NBP2-36525] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PGRMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: PGRMC1 Antibody [NBP2-36525]

Immunoprecipitation: PGRMC1 Antibody [NBP2-36525]

Immunoprecipitation: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. For blotting immunoprecipitated PGRMC1,it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PGRMC1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-12% Bis-Tris gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PGRMC1

Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney.

Long Name

Progesterone Receptor Membrane Component 1

Alternate Names

HPR6.6, MPR, Progesterone Binding Protein

Gene Symbol

PGRMC1

UniProt

NP_006658.1 (Human)

Additional PGRMC1 Products

Product Documents for PGRMC1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGRMC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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