Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PHC3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human polyhomeotic like 3 using the numbering given in entry AAI31773.1 (GeneID 80012).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHC3 Antibody - BSA Free

Western Blot: PHC3 Antibody [NB100-77312]

Western Blot: PHC3 Antibody [NB100-77312]

Western Blot: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: PHC3 Antibody [NB100-77312]

Western Blot: PHC3 Antibody [NB100-77312]

Western Blot: PHC3 Antibody [NB100-77312] - Detection of Human PHC3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PHC3 was also immunoprecipitated by another rabbit anti-PHC3 antibody, which recognizes a downstream epitope.
Immunoprecipitation: PHC3 Antibody [NB100-77312]

Immunoprecipitation: PHC3 Antibody [NB100-77312]

Immunoprecipitation: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for IP at 3 ug per reaction. PHC3 was also immunoprecipitated by rabbit anti-PHC3 antibody NB100-77313. For blotting immunoprecipitated PHC3, NB100-77312 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PHC3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHC3

Polyhomeotic-like 3 (PHC3) is a member of the polycomb family and part of the human polycomb complex, hPRC-H, a complex that represses HOX gene expression throughout development.

Alternate Names

DKFZp313K1221, early development regulator 3, Early development regulatory protein 3, EDR3FLJ12729, FLJ12967, Homolog of polyhomeotic 3, hPH3, MGC88144, PH3, polyhomeotic homolog 3 (Drosophila), polyhomeotic like 3, polyhomeotic like 3 (Drosophila), polyhomeotic-like protein 3

Entrez Gene IDs

80012 (Human)

Gene Symbol

PHC3

UniProt

AAI31773.1 (Human)

Additional PHC3 Products

Product Documents for PHC3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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