PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22531

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22531-100ul
NBP3-22531-50ul
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1816

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PHD4/HIF Prolyl Hydroxylase 4 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PHD4/HIF Prolyl Hydroxylase 4 (Uniprot #: Q9NXG6)

Specificity

Detects endogenous levels of total PHD4/HIF Prolyl Hydroxylase 4

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816)

Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816) [NBP3-22531] -

Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816) [NBP3-22531] - Western blot analysis using NBP3-22531 in HeLa cell lysate.

Applications for PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816)

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHD4/HIF Prolyl Hydroxylase 4

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Alternate Names

EC 1.14.11.-, EGLN4, HIFPH4, HIF-PH4, HIF-prolyl hydroxylase 4, HPH-4, hypoxia-inducible factor prolyl 4-hydroxylase, Hypoxia-inducible factor prolyl hydroxylase 4, P4H with transmembrane domain, P4H-TMFLJ20262, PH-4, PH4 hypoxia inducible factor prolyl 4 hydroxylase, PHD4, proline 4-hydroxylase, prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum), Prolyl hydroxlase domain-containing 4, transmembrane prolyl 4-hydroxylase

Gene Symbol

P4HTM

Additional PHD4/HIF Prolyl Hydroxylase 4 Products

Product Documents for PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHD4/HIF Prolyl Hydroxylase 4 Antibody (SR1816)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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