Phenylalanine Hydroxylase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80917

Novus Biologicals, part of Bio-Techne
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NBP1-80917
NBP1-80917-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Phenylalanine Hydroxylase Antibodies »

Product Summary for Phenylalanine Hydroxylase Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPG

Reactivity Notes

Mouse (86%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phenylalanine Hydroxylase Antibody

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917]

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917]

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917]

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917]

Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex, kidney, liver and pancreas using Anti-Phenylalanine Hydroxylase antibody NBP1-80917 (A) shows similar protein distribution across tissues to independent antibody NBP2-48615 (B).
Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-80917]

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-80917]

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis using Anti-PAH antibody NBP1-80917 (A) shows similar pattern to independent antibody NBP2-48615 (B).
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Applications for Phenylalanine Hydroxylase Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phenylalanine Hydroxylase

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Alternate Names

EC 1.14.16, EC 1.14.16.1, PH, Phe-4-monooxygenase, phenylalanine 4-monooxygenase, phenylalanine hydroxylase, phenylalanine-4-hydroxylase, PKU, PKU1

Entrez Gene IDs

5053 (Human)

Gene Symbol

PAH

UniProt

P00439 (Human)

Additional Phenylalanine Hydroxylase Products

Product Documents for Phenylalanine Hydroxylase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phenylalanine Hydroxylase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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