PHF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82613

Novus Biologicals, part of Bio-Techne
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NBP1-82613
NBP1-82613-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ACTQCLSKPLLYGDRFYEFECCVCRGGPEKVRRLQLRWVDVAHLVLYHLSVCCKKKYFDFDREILPFTSENWDSLLLGELSDTPKGERSSKLLSALNSHKDRFISGREIKKRKCLFGLHARMPPPVEPPTGDGALTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF1 Antibody

Western Blot: PHF1 Antibody [NBP1-82613]

Western Blot: PHF1 Antibody [NBP1-82613]

Western Blot: PHF1 Antibody [NBP1-82613] - Analysis in human testis tissue.
Immunocytochemistry/ Immunofluorescence: PHF1 Antibody [NBP1-82613]

Immunocytochemistry/ Immunofluorescence: PHF1 Antibody [NBP1-82613]

Immunocytochemistry/Immunofluorescence: PHF1 Antibody [NBP1-82613] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry: PHF1 Antibody [NBP1-82613]

Immunohistochemistry: PHF1 Antibody [NBP1-82613]

Immunohistochemistry: PHF1 Antibody [NBP1-82613] - Imaging PHF-1 expression in prostate cancer. Image submitted by a verified customer review.
View 1 more images

Applications for PHF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. PHF1 antibody validated for IHC from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-82613 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF1

PHF1 encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.

Alternate Names

hPCl1, MTF2L2, PCL1, PHD finger protein 1, PHF2, Polycomb-like protein 1, Protein PHF1

Gene Symbol

PHF1

Additional PHF1 Products

Product Documents for PHF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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