PHF23 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28747
![Western Blot: PHF23 Antibody [NBP1-28747] Western Blot: PHF23 Antibody [NBP1-28747]](https://resources.bio-techne.com/images/products/PHF23-Antibody-Western-Blot-NBP1-28747-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 353 and 403 of human PHD finger protein 23 using the numbering given in entry NP_077273.2 (GeneID 79142).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHF23 Antibody - BSA Free
Western Blot: PHF23 Antibody [NBP1-28747]
Western Blot: PHF23 Antibody [NBP1-28747] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHF23 antibody NBP1-28747 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. PHF23 was also immunoprecipitated by rabbit anti-PHF23 antibody NBP1-28746, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Immunohistochemistry: PHF23 Antibody [NBP1-28747]
Immunohistochemistry: PHF23 Antibody [NBP1-28747] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PHF23 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.Applications for PHF23 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2,000
Immunohistochemistry-Paraffin
1:500 - 1:2,000
Immunoprecipitation
10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PHF23
Alternate Names
FLJ16355, FLJ22884, hJUNE-1b, MGC2941, PDH-containing protein JUNE-1, PHD finger protein 23, PHD finger protein 23a
Entrez Gene IDs
79142 (Human)
Gene Symbol
PHF23
UniProt
Additional PHF23 Products
Product Documents for PHF23 Antibody - BSA Free
Product Specific Notices for PHF23 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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