PHF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68261

Novus Biologicals, part of Bio-Techne
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NB100-68261
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Single Cell Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PHF6 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human PHD finger protein 6 using the numbering given in entry NP_115834.1 (GeneID 84295).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF6 Antibody

Western Blot: PHF6 Antibody [NB100-68261]

Western Blot: PHF6 Antibody [NB100-68261]

Western Blot: PHF6 Antibody [NB100-68261] - Detection of Human PHF6 on HeLa whole cell lysate using NB100-68261. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68262.
Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68261]

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68261]

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68261] - Section of human breast carcinoma. Affinity purified rabbit anti- PHF6 used at 1:200 (1 ug/mL). Detection by DAB.

Applications for PHF6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2 - 5 ug/mg lysate

Single Cell Western

100 ug/mL

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Single Cell Western reported by an internal validation on treated IMR32 cells at a concentration of 100ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHF6

This gene is a member of the plant homeodomain (PHD)like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

BFLS, BORJ, Borjeson-Forssman-Lehmann syndrome, KIAA1823, MGC14797, PHD finger protein 6, PHD-like zinc finger protein

Entrez Gene IDs

84295 (Human); 70998 (Mouse)

Gene Symbol

PHF6

UniProt

Q8IWS0 (Human)

Additional PHF6 Products

Product Documents for PHF6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PHF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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