PHF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88646

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-88646
NBP1-88646-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHF6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDVQKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF6 Antibody

Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646]

Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646]

Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining in human epididymis and liver tissues using anti-PHF6 antibody. Corresponding PHF6 RNA-seq data are presented for the same tissues.
Western Blot: PHF6 Antibody [NBP1-88646]

Western Blot: PHF6 Antibody [NBP1-88646]

Western Blot: PHF6 Antibody [NBP1-88646] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: PHF6 Antibody [NBP1-88646]

Immunocytochemistry/ Immunofluorescence: PHF6 Antibody [NBP1-88646]

Immunocytochemistry/Immunofluorescence: PHF6 Antibody [NBP1-88646] - Staining of human cell line U-251MG shows positivity in nucleus and nucleoli. Antibody staining is shown in green.
View 5 more images

Applications for PHF6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 -1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF6

PHF6 is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

BFLS, BORJ, Borjeson-Forssman-Lehmann syndrome, KIAA1823, MGC14797, PHD finger protein 6, PHD-like zinc finger protein

Gene Symbol

PHF6

Additional PHF6 Products

Product Documents for PHF6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.