PHF7 Antibody (3F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00051533-M13

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00051533-M13
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHF7 Antibodies »

Product Specifications

Immunogen

PHF7 (NP_057567.3, 258 a.a. ~ 357 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GRDSFEDEGRWCLILCATCGSHGTHRDCSSLRSNSKKWECEECSPAAATDYIPENSGDIPCCSSTFHPEEHFCRDNTLEENPGLSWTDWPEPSLLEKPES

Specificity

PHF7 - PHD finger protein 7 (3F3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PHF7 Antibody (3F3)

Western Blot: PHF7 Antibody (3F3) [H00051533-M13]

Western Blot: PHF7 Antibody (3F3) [H00051533-M13]

Western Blot: PHF7 Antibody (3F3) [H00051533-M13] - Analysis of PHF7 expression in transfected 293T cell line by PHF7 monoclonal antibody (M13), clone 3F3. Lane 1: PHF7 transfected lysate (Predicted MW: 43.8 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: PHF7 Antibody (3F3) [H00051533-M13] - Detection limit for recombinant GST tagged PHF7 is 0.1 ng/ml as a capture antibody.

Applications for PHF7 Antibody (3F3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHF7

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Alternate Names

DKFZp434L1850, HSPC045, HSPC226, MGC26088, NYD-SP6, PHD finger protein 7, Testis development protein NYD-SP6

Entrez Gene IDs

51533 (Human)

Gene Symbol

PHF7

UniProt

NP_057567 (Human)

Additional PHF7 Products

Product Documents for PHF7 Antibody (3F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHF7 Antibody (3F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.