PHF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93313

Novus Biologicals, part of Bio-Techne
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NB100-93313
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation Sequencing, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PHF8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human PHD finger protein 8 (CAI41576.1).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF8 Antibody

Western Blot: PHF8 Antibody [NB100-93313]

Western Blot: PHF8 Antibody [NB100-93313]

Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313]

Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313]

Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Western Blot: PHF8 Antibody [NB100-93313]

Western Blot: PHF8 Antibody [NB100-93313]

Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93313. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93314.
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Applications for PHF8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100- 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
ChIP-Seq 10 ug as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHF8

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Long Name

Histone lysine demethylase PHF8

Alternate Names

KIAA1111, ZNF422

Entrez Gene IDs

23133 (Human)

Gene Symbol

PHF8

UniProt

Q9UPP1 (Human)

Additional PHF8 Products

Product Documents for PHF8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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