PHF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62797

Novus Biologicals, part of Bio-Techne
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NBP2-62797
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PHF8 Antibodies »

Product Summary for PHF8 Antibody

Immunogen

Human PHF8 (PHD finger protein 8)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF8 Antibody

Western Blot: PHF8 Antibody [NBP2-62797]

Western Blot: PHF8 Antibody [NBP2-62797]

Western Blot: PHF8 Antibody [NBP2-62797] - E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed using the antibody against PHF8.
ELISA: PHF8 Antibody [NBP2-62797]

ELISA: PHF8 Antibody [NBP2-62797]

ELISA: PHF8 Antibody [NBP2-62797] - To determine the titer of the antibody, an analysis was performed using a serial dilution of the antibody directed against human PHF8, crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:6,500.

Applications for PHF8 Antibody

Application
Recommended Usage

ELISA

1:100 - 1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PHF8

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Long Name

Histone lysine demethylase PHF8

Alternate Names

KIAA1111, ZNF422

Gene Symbol

PHF8

Additional PHF8 Products

Product Documents for PHF8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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