PHGDH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87310

Novus Biologicals, part of Bio-Techne
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NBP1-87310
NBP1-87310-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHGDH Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHGDH Antibody

Western Blot: PHGDH Antibody [NBP1-87310]

Western Blot: PHGDH Antibody [NBP1-87310]

Western Blot: PHGDH Antibody [NBP1-87310] - Analysis using Anti-PHGDH antibody NBP1-87310 (A) shows similar pattern to independent antibody NBP1-87311 (B).
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-87310]

Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-87310]

Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-87310] - Staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
View 2 more images

Applications for PHGDH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHGDH

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Long Name

3-Phosphoglycerate Dehydrogenase

Alternate Names

3PGDH, PDGH3

Gene Symbol

PHGDH

Additional PHGDH Products

Product Documents for PHGDH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHGDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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