PHIP Antibody (4D7)

Novus Biologicals, part of Bio-Techne | Catalog # H00055023-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4D7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHIP Antibodies »

Product Specifications

Immunogen

PHIP (NP_060404, 1599 a.a. ~ 1705 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLSKSSAVIEQGDCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDL

Specificity

PHIP - pleckstrin homology domain interacting protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PHIP Antibody (4D7)

Immunocytochemistry/ Immunofluorescence: PHIP Antibody (4D7) [H00055023-M01]

Immunocytochemistry/ Immunofluorescence: PHIP Antibody (4D7) [H00055023-M01]

Immunocytochemistry/Immunofluorescence: PHIP Antibody (4D7) [H00055023-M01] - Analysis of monoclonal antibody to PHIP on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: PHIP Antibody (4D7) [H00055023-M01]

ELISA: PHIP Antibody (4D7) [H00055023-M01]

ELISA: PHIP Antibody (4D7) [H00055023-M01] - Detection limit for recombinant GST tagged PHIP is 0.1 ng/ml as a capture antibody.

Applications for PHIP Antibody (4D7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It is also useful for immunofluorescence.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHIP

PH-interacting protein (PHIP) was isolated as factor that binds to the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS-1). PHIP was determined not to be a substrate of the insulin receptor but may instead serve to link IRS-1 to the insulin receptor. PHIP has also been implicated as a positive regulator of beta-cell mitogenesis and survival. PHIP is also known as WD-repeat-containing protein 11, WDR11, and IRS-1 PH domain-binding protein.

Alternate Names

DCAF14, DDB1 and CUL4 associated factor 14, FLJ20705, FLJ45918, IRS-1 PH domain-binding protein, MGC90216, ndrp, PH-interacting protein, pleckstrin homology domain interacting protein, WD repeat-containing protein 11, WDR11

Entrez Gene IDs

55023 (Human)

Gene Symbol

PHIP

UniProt

NP_060404 (Human)

Additional PHIP Products

Product Documents for PHIP Antibody (4D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHIP Antibody (4D7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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