PHIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33883

Novus Biologicals, part of Bio-Techne
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NBP2-33883
NBP2-33883-25ul
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHIP Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDLLQKKNRGGRKPKRKMKTQKLDADLLVPASVKVLR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHIP Antibody

Immunocytochemistry/ Immunofluorescence: PHIP Antibody [NBP2-33883]

Immunocytochemistry/ Immunofluorescence: PHIP Antibody [NBP2-33883]

Immunocytochemistry/Immunofluorescence: PHIP Antibody [NBP2-33883] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
PHIP Antibody

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
PHIP Antibody

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
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Applications for PHIP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHIP

PH-interacting protein (PHIP) was isolated as factor that binds to the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS-1). PHIP was determined not to be a substrate of the insulin receptor but may instead serve to link IRS-1 to the insulin receptor. PHIP has also been implicated as a positive regulator of beta-cell mitogenesis and survival. PHIP is also known as WD-repeat-containing protein 11, WDR11, and IRS-1 PH domain-binding protein.

Alternate Names

DCAF14, DDB1 and CUL4 associated factor 14, FLJ20705, FLJ45918, IRS-1 PH domain-binding protein, MGC90216, ndrp, PH-interacting protein, pleckstrin homology domain interacting protein, WD repeat-containing protein 11, WDR11

Gene Symbol

PHIP

UniProt

Q8WWQ0 (Human)

Additional PHIP Products

Product Documents for PHIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PHIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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