PHLDA1 Antibody

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells as expected.

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse and subjected to heat shock after 2, 4, and 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids and condensing spermatids are marked with arrowhead and arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906015/), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] -

Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. (a) Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse & subjected to heat shock after 2, 4, & 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids & condensing spermatids are marked with arrowhead & arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. (b) Detection of apoptotic DNA breaks (by TUNEL assay, red; DNA stained with DAPI, blue) in seminiferous tubules of untreated mice & after heat shock in vivo & indicated recovery time (2–6 h). (c) Detection of PHLDA1 by immunofluorescence (red; DNA stained with DAPI, blue) in cryptorchid testes 7 & 14 days after surgery (upper panel) & detection of apoptotic DNA breaks (by TUNEL assay, red) in corresponding tissues (bottom panel). Scale bar—50 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PHLDA1 Antibody [NBP1-84969] -

Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PHLDA1 Antibody [NBP1-84969] -

Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.