PHLPP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13757

Novus Biologicals, part of Bio-Techne
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NBP2-13757
NBP2-13757-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PHLPP2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: DLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHLPP2 Antibody

Immunocytochemistry/ Immunofluorescence: PHLPP2 Antibody [NBP2-13757]

Immunocytochemistry/ Immunofluorescence: PHLPP2 Antibody [NBP2-13757]

Immunocytochemistry/Immunofluorescence: PHLPP2 Antibody [NBP2-13757] - Staining of human cell line HEK 293 shows localization to vesicles.
Immunohistochemistry-Paraffin: PHLPP2 Antibody [NBP2-13757]

Immunohistochemistry-Paraffin: PHLPP2 Antibody [NBP2-13757]

Immunohistochemistry-Paraffin: PHLPP2 Antibody [NBP2-13757] - Staining of human spleen shows strong positivity in a subset of cells in the red pulp.

Applications for PHLPP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHLPP2

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Alternate Names

EC 3.1.3.16, KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase-like, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPPLPHLPP-like

Gene Symbol

PHLPP2

Additional PHLPP2 Products

Product Documents for PHLPP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHLPP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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